On Jan 4, 6:38 am, hamze rahimi <buksan... from gmail.com> wrote:
> Dear expert
>> I download blast-2.2.18-ia32-linux.tar.gz and unzipped. when I want run
> under command, I find this error
>> [buksanbio from localhost bin]$ ./blastpgp -v 5000 -b 0 -j 3 -h 0.002 -d
> /home/buksanbio/nr/ -i 535.fasta -C.Chk > /dev/null
> [blastpgp] WARNING: Unable to open .pin
>> or
>> [buksanbio from localhost bin]$ ./blastpgp -v 5000 -b 0 -j 3 -h 0.002 -d ~/nr/*
> -i 535.fasta -C.Chk > /dev/null
> [blastpgp] ERROR: Arguments must start with '-' (the offending argument #11
> was: '/home/buksanbio/nrfilt/nr.00.pin')
>> as recommanded in doc file I convert database by this command:
> ./formatdb -i ~/.../nr -p T -o T
>> nr is my database that contain these files:
> nr.00.phr nr.00.psd nr.01.pin nr.01.psi nr.02.pnd nr.02.psq nr.03.pni
> nr.04.phr nr.04.psd
> nr.00.pin nr.00.psi nr.01.pnd nr.01.psq nr.02.pni nr.03.phr nr.03.psd
> nr.04.pin nr.04.psi
> nr.00.pnd nr.00.psq nr.01.pni nr.02.phr nr.02.psd nr.03.pin nr.03.psi
> nr.04.pnd nr.04.psq
> nr.00.pni nr.01.phr nr.01.psd nr.02.pin nr.02.psi nr.03.pnd nr.03.psq
> nr.04.pni nr.pal
>> what is problem?
> thanks a lot for any help
>> --
> Yours sincerely
> Rahimi Hamzeh
> PhD student in Medical Biotechnology
> Pasteur institute
> e mail:
>buksan... from gmail.com
nr is not a directory but the base name of your database. so -d ~/nr/
would not work but ~/nr would. Alternately indicate in .ncbirc where
your formatted databases are located.
D