[Bio-software] blastall query

Victoria D Bohler via bio-soft%40net.bio.net (by vdbohler from gmail.com)
Tue Jul 5 07:04:09 EST 2011


Hello,

In my opinion your database is not in blastable format. You have to use
formatdb command:

http://goo.gl/Vy114

I hope will be useful,

Victoria Dominguez
ABgenomica


On Tue, Jul 5, 2011 at 11:08 AM, ishabioinfo <srivastavaisha.06 from gmail.com>wrote:

> hii
>
> i have downloaded the latest version of local blast  ncbi-
> blast-2.2.25+.
> created .ncbirc file .
> downloaded nr database.
> when i m giving the command -
>
> blastall -p blastp -i mixo2.fasta -d nr -o fasta.out
>
> it is showing the error ---
>
>  "Could not calculate ungapped Karlin-Altschul parameters due to an
> invalid query sequence or its translation. Please verify the query
> sequence(s) and/or filtering options"
>
> kindly help me to solve this problem
>
> thanku :)
>
>
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