Gene Quantification Newsletter - 10/2002

Gene Quantification Homepage gene.quantification at wzw.tum.de
Thu Oct 24 06:06:00 EST 2002


Gene Quantification Newsletter - 10/2002
October 2002

Introduction:
Main focus of the GENE QUANTIFICATION web 
page   http://www.wzw.tum.de/gene-quantification/   is, to describe and 
summarize all technical aspects involved in quantitative gene expression 
analysis using real-time (kinetic) PCR  &  RT-PCR:  e.g. detection 
chemistry, assay specificity, sensitivity, reproducibility, robustness, 
intra- & inter-assay variations, kinetic PCR efficiency calculation, 
quantification strategies, optimisation strategy, data calculation and 
statistics,  etc. etc.

The GENE QUANTIFICATION web page illustrates the usefulness of reliable 
quantification strategies, e.g. absolute-, relative-quantification assays, 
and the difference between them in kinetic PCR & kinetic RT-PCR.

Main focus of the PHYSIOLOGY web page is, to describe all kind of kinetic 
RT-PCR applications in molecular Physiology, molecular Endocrinology, and 
molecular Immunology (mainly mammary gland immunology) are summarized on my 
new homepage   http://www.wzw.tum.de/gene-quantification/physiology.shtml

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NEW features:

Determination of real-time PCR efficiency  -  NEW EQUATIONS
http://www.wzw.tum.de/gene-quantification/efficiency.html


Endocrinology & Mammary Gland Immunology  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/physiology.shtml
Steroid receptors:   AR, ERa, ERb, PR
Adrenergic 
receptors:   alpha-1-subtypes  &  alpha-2-subtypes  &  beta-subtypes 
(publications in press)
Somatotropic axis:   GHR, IGF-1, IGF2, both IGF receptors  & IGF-BP1 to BP3
Serotonin receptors   (coming soon)

NEW MOVIES
http://www.wzw.tum.de/gene-quantification/movie.html
e.g.  PCR, RPA, Electrophoresis, Northern- & Southern-Blot


NEW PUBLICATIONS about kinetic PCR
http://www.wzw.tum.de/gene-quantification/real-time.html
and
http://www.wzw.tum.de/gene-quantification/chemistry.html
and
http://www.wzw.tum.de/gene-quantification/hkg.html
and
http://www.wzw.tum.de/gene-quantification/literature.html
sections:
      RT-PCR and real-time RT-PCR (in general)
      LightCycler specific technical notes
      absolute quantification using kinetic PCR
      relative quantification using kinetic PCR
      classical block RT-PCR   &   competitive RT-PCR
      normalisation  &  housekeeping gene problems in real-time PCR
      verification of DNA array results
      quantification software: REST,    REST-XL,   Q-Gene,   geNorm
      kinetic  RT-PCR  poster board


Poster boards (a little bit slow !!!):
http://www.wzw.tum.de/gene-quantification/poster-a.html
methodological  poster board A:  Array verification
physiological  poster board B:     IGFs - Early Response Genes
physiological  poster board C:     Steroid receptors
physiological  poster board D:     Zinc metabolism
physiological  poster board E:     Immunological relevant factors

New links & databases:
http://www.wzw.tum.de/gene-quantification/link.html
e.g.
-  Dictionary of Nucleic Acid Structures (DNAS)
-  Glossary iSenseIT
-  Molecular Biology Glossary
-  A Hypermedia Glossary of Genetic Terms pick from an alphabetical list of 
Terms with 656 entries
http://www.realtimeprimerdatabase.ht.st
http://www.realtimeprimers.org/
http://www.pcrlinks.com
http://www.cyclertest.com/
The COMPLETE Alkami Quick GuideTM for PCR is a (158 pages)
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
LightCycler University (LCU)
http://www.wzw.tum.de/gene-quantification/real-time.html#lcu


Please send the NEWSLETTER and the home page links to further scientists 
and friends who are interested in real-time PCR, new quantification 
strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

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For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE 
to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact 
gene.quantification at wzw.tum.de   You will receive all NEW FEATURES and 
future REST &  REST-XL updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are 
non-commercial, educational sites with the only purpose of facilitating 
access to biology-related information over the internet. The web page is 
optimised for Netscape Navigator version 4.7 & Internet Explorer version 
5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 
pixels.

GENE QUANTIFICATION web page visits and DOWNLOAD statistics are listed 
here: http://www.wzw.tum.de/gene-quantification/contact.html#server

If you want UN-SUBSCRIBE from all future NEWSLETTERS please contact 
gene.quantification at wzw.tum.de and type REMOVE into the subject line. You 
will be removed instantly from our mailing list.



********************************************************************
Gene Quantification @ wzw.tum.de

e-mail: gene.quantification at wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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