Eastern 2003 - Newsletter

Gene Quantification Homepage gene.quantification at wzw.tum.de
Tue Apr 22 03:49:19 EST 2003


Gene Quantification Newsletter  Eastern 2003


Happy Eastern to all my visitors and software users !!!
We are happy to welcome the 65,000th visitor on my Gene Quantification Pages
During the last 14 months !!!

Thank you very much !!!

New features:

A lot of NEW real-time PCR workshops
http://www.wzw.tum.de/gene-quantification/main.shtml#work

A seminar in the calculation and normalization of real-time RT-PCR results
http://folk.uio.no/kristban/realtime/index.html

2nd Nucleic Acid Quantification Meeting  in  London, UK
http://www.mds.qmw.ac.uk/surgery/events/Announcement.html

Amplifications for real-time qPCR Analysis workshop
https://dynamic.mjr.com/workshop/home.jsp

Quantitative real-time PCR course at RoboScreen
http://www.roboscreen.com/home/frame.htm

etc.   etc.   etc.


New Section BioInformatics in real-time PCR

http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml

BestKeeper© software - Determination of most stable housekeeping genes, 
differentially regulated target genes and template 
integrity:  BestKeeper©    Excel © spreadsheet tool using a Multiple 
Correlation and Regression Analysis
http://www.wzw.tum.de/gene-quantification/bestkeeper.html

Determination of most stable housekeeping genes, differentially regulated 
target genes and sample integrity: BestKeeper© - Excel© spreadsheet tool 
using a Repeated Pair-wise Correlation and Regression Analysis
21. Dechema Jahrestagung der Biotechnologen 2.-4. April 2003
http://www.wzw.tum.de/gene-quantification/poster-f.html

LinRegPCR  -  Assumption-free analysis of quantitative real-time PCR data
http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-4

Determination of real-time PCR efficiency
http://www.wzw.tum.de/gene-quantification/efficiency.html

NEW EQUATIONS & ALGORITHM by Tichopad et al. 2002
http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2


NEW PUBLICATIONS about kinetic PCR
http://www.wzw.tum.de/gene-quantification/real-time.html
and
http://www.wzw.tum.de/gene-quantification/literature.html


Gene Quantification in Endocrinology & Immunology

Tissue-specific expression pattern of bovine prion gene 
(PrP):  Quantification using real-time RT-PCR
http://www.wzw.tum.de/gene-quantification/absolute.html#prp

Detection and quantification of mRNA-expression of alpha- and beta-adrenergic
receptor subtypes in the mammary gland of dairy cows.
http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg

The gastrointestinal tract as target of steroid hormone action: 
Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta 
and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR
http://www.wzw.tum.de/gene-quantification/endo.html#erab

Characterisation of gene expression patterns in 22RV1 cells for 
determination of environmental androgenic / antiandrogenic compounds
http://www.wzw.tum.de/gene-quantification/endo.html#compounds

Gene Expression of Immunologically Important Factors in Blood Cells, Milk 
Cells and Mammary Tissue of Cows.
http://www.wzw.tum.de/gene-quantification/immuno.html#papers

Insulin-like growth factor and insulin receptors in intestinal mucosa of 
neonatal calves.
http://www.wzw.tum.de/gene-quantification/endo.html#igfs


Please send the NEWSLETTER and the home page links to further scientists 
and friends who are interested in real-time PCR, new quantification 
strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

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future REST &  REST-XL  &  BestKeeper   updates.

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********************************************************************
Gene Quantification @ wzw.tum.de

e-mail: gene.quantification at wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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