quantitative PCR News Dec. 2003

qPCR 2004 Symposium - Gene Quantification Newsletter gene.quantification at wzw.tum.de
Mon Dec 8 09:01:42 EST 2003


Gene Quantification Newsletter December 2003

New features on the Gene-Quantification web page:

Influence of zinc deficiency on the mRNA expression of zinc transporters in 
adult rats.
<http://www.wzw.tum.de/gene-quantification/zinc.html>http://www.wzw.tum.de/gene-quantification/zinc.html


The mRNA expression of the members of the IGF-system in bovine corpus 
luteum during induced luteolysis

Abundance of message for insulin-like growth factors-1 and -2 and for 
receptors for growth hormone, insulin-like growth factors-1 and -2, and 
insulin in the intestine and liver of pre- and full-term calves.

Effects of dexamethasone and colostrum intake on the somatotropic axis in 
neonatal calves.

The mRNA expression of the members of the IGF-system in bovine corpus 
luteum during induced luteolysis.
<http://www.wzw.tum.de/gene-quantification/endo.html#igfs>http://www.wzw.tum.de/gene-quantification/endo.html#igfs


Expression and localisation of oestrogen and progesterone receptors in the 
bovine mammary gland during development, function and involution.

The gastrointestinal tract as target of steroid hormone action: 
Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta 
and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR.
<http://www.wzw.tum.de/gene-quantification/endo.html#erab>http://www.wzw.tum.de/gene-quantification/endo.html#erab


Detection and quantification of mRNA-expression of alpha- and 
beta-adrenergic receptor subtypes in the mammary gland of dairy cows.

Milking characteristics and their relation to adrenergic receptor mRNA 
expression and ligand binding in the mammary gland of dairy cows.
<http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg>http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg




New Section BioInformatics in real-time PCR

<http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml>http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml

  Determination of real-time PCR efficiency
<http://www.wzw.tum.de/gene-quantification/efficiency.html>http://www.wzw.tum.de/gene-quantification/efficiency.html

  PCR Efficiency estimation via "experimental four parametric logistic 
model fit" (including all fluorescence data points, Tichopad et al. 2003 - 
Standardized determination of real-time PCR efficiency from a single 
reaction set-up.)
<http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-3>http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-3

  Erratum of Figure 2 Tichopad et al. 2003 
<http://www.wzw.tum.de/gene-quantification/efficiency.html#correction>http://www.wzw.tum.de/gene-quantification/efficiency.html#correction

Improving quantitative real-time RT-PCR reproducibility by boosting 
primer-linked amplification efficiency
<http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2>http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2

  KOD (kinetic outlier detection) in real-time PCR (Tzachi Bar et al., 2003)
<http://www.wzw.tum.de/gene-quantification/efficiency.html#kod>http://www.wzw.tum.de/gene-quantification/efficiency.html#kod





NEW PUBLICATIONS about kinetic PCR

<http://www.wzw.tum.de/gene-quantification/real-time.html>http://www.wzw.tum.de/gene-quantification/real-time.html
and
<http://www.wzw.tum.de/gene-quantification/literature.html>http://www.wzw.tum.de/gene-quantification/literature.html

Please send the NEWSLETTER and the home page links to further scientists 
and friends who are interested in real-time PCR, new quantification 
strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

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1st International qPCR Symposium  &  Application Workshop
Transcriptomics, Clinical Diagnostics  &  Gene Quantification
3rd - 6th March, 2004 in Freising-Weihenstephan, Germany
<http://www.wzw.tum.de/gene-quantification/qpcr2004/index.shtml>http://www.wzw.tum.de/gene-quantification/qpcr2004/index.shtml

  Up to now around 150 Abstracts submitted from 31 Nations worldwide
<http://www.wzw.tum.de/gene-quantification/qpcr2004/main-2004.shtml#news>http://www.wzw.tum.de/gene-quantification/qpcr2004/main-2004.shtml#news

  The Symposium and the Application Workshop presents unbiased 
international lectures & posters about quantification strategies, 
normalisation methods & new algorithms for exact nucleic acid 
quantification in the broad range of qPCR applications: Transcriptomics, 
Clinical Diagnostics, Food Hygiene  &  GMO, Expression profiling, 
Microbiology, Pharmacogenomics, Forensic, Nutrigenomics, Molecular 
Endocrinology, cDNA Array Verification, Theoretical qPCR, Pre-analytical 
Steps  &  RT, Isolation of Nucleic Acids.
<http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-workshop.html>http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-workshop.html

More than 40 internationally renown speakers will be participating in a 
lively and exciting programme enabling the valuable exchange of information 
in the qPCR field. As a special guest we can announce Prof. Carl Wittwer 
from the Institute of pathology at the University of Utah School of 
Medicine. He is an associate editor of Clinical Chemistry, and best known 
for developing techniques in rapid-cycle PCR and as the inventor of the 
LightCycler.
<http://www.wzw.tum.de/gene-quantification/qpcr2004/main-2004.shtml#speaker>http://www.wzw.tum.de/gene-quantification/qpcr2004/main-2004.shtml#speaker

  Parallel to the Symposium an Industrial Exhibition will take place along 
with the congress, where an intensive conversation with numerous companies 
is possible (real-time PCR Cyclers, Kit Producers, Nucleic Acid 
Purification Systems, Enzyme Producers, Plastic ware Suppliers, etc.). 25 
companies agreed to participate at the Industrial Exhibition.
<http://www.wzw.tum.de/gene-quantification/qpcr2004/companies.html>http://www.wzw.tum.de/gene-quantification/qpcr2004/companies.html




The organization team thank all SPONSORS of the Symposium:

<http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-sponsors.html>http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-sponsors.html

Platinum Sponsor Roche Diagnostics

Gold Sponsor BioRad

Gold Sponsor MJ Research

Silver Sponsor ABI Applied Biosystems

Silver Sponsor Invitrogen

Silver Sponsor Qiagen

Silver Sponsor Stratagene


  ################################

Symposium organization & registration:

Registration Form 
(PDF) 
<http://www.wzw.tum.de/gene-quantification/qpcr2004/registration.pdf>http://www.wzw.tum.de/gene-quantification/qpcr2004/registration.pdf

Registration Form 
(DOC) 
<http://www.wzw.tum.de/gene-quantification/qpcr2004/registration.doc>http://www.wzw.tum.de/gene-quantification/qpcr2004/registration.doc


********************************************************************************************
Gene.Quantification @ wzw.tum.de

e-mail:         gene.quantification at wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml

responsible Senior Scientist:

PD Dr. Michael W. Pfaffl
Physiology - Weihenstephan
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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