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Announcement: the Lipase Engineering Database

Juergen Pleiss jpleiss at tebio1.biologie.uni-stuttgart.de
Fri Feb 4 11:57:43 EST 2000

The Lipase Engineering Database is online !

The Lipase Engineering Database (LED) is a WWW-accessible resource on sequence-structure-function 
relationships of microbial lipases. Sequences of 92 microbial lipases and homologous serine 
hydrolases were assigned to 32 homologous families and 15 superfamilies. Multisequence 
alignments of all homologous families and superfamilies are provided. Functionally relevant
amino acids are annotated. The complete catalytic machinery (catalytic triad and two oxyanion 
hole residues) could be annotated in 91 % of LED sequence entries. Published mutants and their 
properties are provided. The X-ray structures of 22 lipases were superposed and annotated. 

Consistently annotated aligned sequences and superimposed structures of microbial lipases help 
to understand the functional role of individual amino acids and thus the LED is a useful tool 
for protein engineering.

We have also set up a mailing list as a forum for discussions and announcements on topics
related to protein engineering of lipases. More specifically we are keen to learn about
the usefulness of the LED and suggestions on how to improve and extend it. What is
missing? Would you like to contribute data?
The LED mailing list is currently an open, unmoderated list. Any postings are automatically accepted.
Subscribers are encouraged to post messages to LED at listserv.uni-stuttgart.de
The list owner is Markus Fischer.

To subscribe to the LED mailing list send the following message to majordomo at listserv.uni-stuttgart.de 
                  subscribe led 
You will receive an e-mail, which will ask you for authentication. 

To unsubscribe send the following message to majordomo at listserv.uni-stuttgart.de 
                  unsubscribe led 

Have fun!

Juergen Pleiss (jpleiss at tebio1.biologie.uni-stuttgart.de)
Markus Fischer (Markus.Fischer at po.uni-stuttgart.de)

Institute of Technical Biochemistry, Stuttgart       Lipase Engineering Database
http://www.itb.uni-stuttgart.de                      http://www.led.uni-stuttgart.de

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