Restriction digest with codon usage redundancy

Gregory Adrianus O'Sullivan osullivan at
Mon Dec 1 05:13:52 EST 2003

Dear Bionetters,

I am trying to introduce restriction sites into a DNA sequence, which do not affect the protein sequence. Is there any program
out there that can do this automatically for me, that is scan all the possible DNA sequece permutations and then generate
an output in which it specficies a 6 cutter that has been newly generated in the sequence for each possibility.


Gregory A. O'Sullivan PhD.
Dept. of Neurochemistry,
Max-Planck-Institute für Hirnforschung,
Deutschordenstrasse 46,
D-60528 Frankfurt/Main,
Tel: +49 69 96769315
Mob: +49 160 2624404
Fax: +49 69 96769441
Email: osullivan at
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