Cell preparation for PCR

greg at MENDEL.LLNL.GOV greg at MENDEL.LLNL.GOV
Tue May 25 12:15:45 EST 1993


The simplest prep is probably just adding TE, and boiling
for 10 minutes.  Many folks then do a "prep-PCR" for 10-20
cycles using random primers, then use aliquots or dilutions
of that PCR for the specific PCRs to follow.

A few references on this or other methods for single cell PCR :

NAR 21,1321-2 (1993)
Genomics 13,718-725 (1992)
Genomics 16,41-44 (1993)
Genomics 10,748-755 (1991)

Hope this helps,

Greg Lennon
Human Genome Center, L-452
Lawrence Livermore National Laboratory
Livermore, CA 94550 USA




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