RARE technique; Rec-A mediated chromosome cleavage
Peter Myler
mylerpj at u.washington.edu
Fri Dec 2 15:06:22 EST 1994
On 1 Dec 1994, John J Weiland wrote:
>
> Can someone please post or mail me a reference for the use of RecA in the
> blocking of methylation sites on DNA? I think the technique is
> called RARE, and was mentioned in the recent Promega Notes with
> regard to their Rec A product. Thanks in advance!
>
> John Weiland jweiland at badlands.nodak.edu
>
>
I believe the reference is:
Ferrin, L.J. and Camerini-Otero, R.D. (1991) Selective cleavage
of human DNA: RecA-assisted restriction enzyme (RARE) cleavage. Science.
254:1494-1497.
Regards
Peter
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Peter J. Myler phone: (206) 284-8846x332
Seattle Biomedical Research Institute FAX: (206) 284-0313
4 Nickerson Street e-mail: MYLERPJ at U.WASHINGTON.EDU
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