ABI Taq Pol sequencing reactions

Mike Morris mike at medsun.unige.ch
Mon Dec 19 07:21:10 EST 1994


  For our ABI373 sequencer, we currently go through the following 
protocol, which gives good consistent results:

	miniprep plasmids
	measure OD
	sequence (Taq Pol, dye primers)
	phenol
	chloroform
	precipitate
	ethanol wash
	run.

  The slowest part, and the bottleneck of the whole protocol, is the
extraction/precipitation, which seriously limits our throughput. 

  Could anybody give us tried-and-tested protocols for speeding this 
up? Even if the cost goes up (I have wondered about spin columns), 
the increase in throughput would make this worthwhile.

  Thanks in advance,
	Mike Morris
*******************************************************************           
Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
CMU, University of Geneva          fax (Switzerland) (22) 702.57.06
Geneva, Switzerland                email mike at medsun.unige.ch                  





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