Quantifying hybridization to arrayed colonies

Steve Clark SCLARK at AMGEN.COM
Thu Jun 16 18:34:36 EST 1994


Hi,

	I'm trying to locate software that can be used to quantify 
hybridization to arrayed colonies that have been spotted, in an interleaved
fashion, on membranes. The colonies will have been spotted on the filter
from 96 well (or denser) microtitre plates in a 3x3 to 5x5 interleaved
pattern (that is, 9 to 25 plates of clones will be spotted on one filter).
The software should be able to read a rasterized image of the autorad,
automatically locate the colonies and write out a file with the colony
positions and the amount of hybridization. If anyone knows of people or 
groups using or developing such software, I would appreciate hearing about 
them. (For hardware, we prefer something that will run on a Mac or Sun, but 
we can handle just about anything.)

	Thanks in advance for the help. I'll summarize the responses
back to the newsgroup.


Steve Clark

sclark at amgen.com



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