lambda Charon 40 in RecA- host

Nancy J. Phillips, M.D. PHILLINJ at SLUVCA.SLU.EDU
Fri Jun 17 18:52:28 EST 1994


Dear netters,
	Does anyone have suggestions on how to get decent titers/DNA
yields with lambda Charon 40 clones in the recA- host K802recA-? I 
have tried liquid lysates with multiplicities of infection from 1:100
to 1:500 and 4 - 12 hour incubations, and media including LB/1mM MgCl2
and LB/2 mM CaCl2/10 mM MgSO4/0.1% glucose.
	Also, is PEG-8000/NaCl precipitation of lambda inherently 
inefficient at low titers? I follow the usual Maniatis protocol.
	Thanks!

Nancy Phillips				phillinj at sluvca.slu.edu
Pathology				phone:(314)577-8782
St. Louis University Hospital		fax:(314)268-5120
3635 Vista Ave.
St. Louis MO 63110 USA




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