Chromosome Harvesting

[Drew Reichelt]

In <35tfja$a9c at pentagon.io.com> buc at pentagon.io.com (Craig  Fox) writes: 

>
>Is anyone out there an expert in chromosome harvest?  
>
>I have had the hardest time trying to get consistent harvests.  Were I 
>work we use a Tecan and a special drying chamber, so all of our sections 
>harvests should be very consistent, but they are not.  
>
>I have noticed that I am getting good spreading under the phase scope 
>but after they are stained the chromosomes are often dark (but not under 
>banded) chewy, split and I have alot of cyto.  I also get alot of nice 
>stuff.  If anyone has had similar problems harvesting and has found a 
>way to get consistently ok to good harvests, please let me know how you 
>did it.
>
>Thanx
>buc
>
>

I have no idea, sorry can't help!
Drew