pcr taq pfuu

[MSLADE at RNA.BIO.MQ.EDU.AU]

We had a singular lack of success getting pfu polymerase to work on its 
own.  As with most problems we have just used the technique that works 
-taq. However, since trying pfu out, I have wondered whether the problem 
could be the level of dNTPs.  Presumably a low level of dNTPs (100uM) 
would bias the reaction towards the nuclease activity rather than the 
polymerase.  Any comments?  or do I need to try higher amounts of dNTPs ?
  1mM?

Cheers, Martin Slade
Martin Slade,
School of Biological Sciences,
Macquarie University,
NSW 2109,
Australia
FAX  (61 2) 850 8174
Phone(61 2) 850 8210