SSLP GENETIC MAP OF THE RAT

Lincoln Stein lstein at genome.wi.mit.edu
Fri Jan 6 18:18:09 EST 1995


    MASSACHUSETTS GENERAL HOSPITAL/MIT CENTER FOR GENOME RESEARCH
			GENETIC MAP OF THE RAT
			      RELEASE 1
			     JANUARY 1995

INTRODUCTION:

This is to announce the January 1995 release of the Massachusetts
General Hospital/Harvard Medical School and Whitehead Institute/MIT
Center for Genome Research rat genetic map.  This map consists of 431
polymorphic rat microsatellite repeats mapped on a SHRxBN F2
intercross using PCR probes.  The average spacing between markers on
these maps is approximately 3.7 cM.

OBTAINING THE DATA:

The data can be obtained as a series of flat files by anonymous ftp to
ftp-genome.wi.mit.edu.  Log in as "anonymous" and give your e-mail
address as your password.  The data files can be found in
/distribution/rat_sslp_releases/janary95.

Alternatively, the data can be found on a World Wide Web site
maintained by the MIT Center for Genome Research.  Point your browser
at http://www-genome.wi.mit.edu/ and follow the links to Genome Center
Data/Rat Genetic Mapping Project.

CONTENTS OF THIS DIRECTORY:

The contents of this directory are as follows:

marker_data.sea.hqx
        A Macintosh Excel 4.0 format file containing data on each of the
        markers, including the sequence of forward and reverse
        primers, the genotype of the markers on the cross, and the
        sizes of the product produced on a number of standard inbred
        strains.

marker_data.txt
        As above in a tab-delimited ASCII text file.

chromosome_pictures/
        A directory containing Macintosh picture files of the maps, one
        per chromosome.

CITING THIS DATA:

References to this data in publications should be cited by listing
the following source:

1. Jacob, H.J., Brown, D.M., Bunker, R.K., Daly, M.J., Dzau, V.J.,
Goodman, A., Koike, G., Kren, V., Kurtz, T., Lernmark, A., Levan, G.,
Mao, Y-P., Pettersson, A., Pravenec, M., Simon, J.S., Szpirer, C.,
Szporer, J., Trolliet, M.R., Winer, E.S. and Lander, E.S., 1995.  A
genetic linkage map of the laboratory rat, Rattus norvegicus, Nature
Genetics 9, 63-69.

ASSAY CONDITIONS:

Assay conditions and other experimental details are given in Jacob,
H.J., et al., 1995.  Nature Genetics 9, 63-69.  Briefly, the PCR
protocol used radiolabeled primers in a 25 cycle PCR (1 '92 degrees,
2' 55 degrees, 1.5' 72 degrees) on 20 ng of genomic DNA.

PURCHASING PRIMER PAIRS:

All the primers listed in this release are available from Research
Genetics, Inc.,2130 Memorial Parkway SW, Huntsville, AL 35801,
800-533-4363 (phone), 800-336-9014 (FAX), under a community discount
arrangement set up by the Massachusetts General Hospital/Harvard
Medical School and the Whitehead Institute/MIT Center for Genome
Research.  (Note: MGH and WI/MIT CGR have placed the markers in the
public domain.  The center and its personnel receive no financial
benefit from the sale of primers.)

FURTHER QUESTIONS:

Please direct questions and inquiries to Donna Brown, telephone 
(617) 726-4347, d_brown at helix.mgh.harvard.edu.

Donna Brown
Massachusetts General Hospital


-- 
========================================================================
Lincoln D. Stein                Whitehead Institute/MIT Genome Center
lstein at genome.wi.mit.edu	Cambridge, MA 02142
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