pcr taq pfu

goldman%bchem.dnet at DXI.NIH.GOV goldman%bchem.dnet at DXI.NIH.GOV
Thu Jan 26 16:57:30 EST 1995


Have tried pfu in straight pcr. it is a very unreliable and fussy enzyme, 
but has the advantage of NO 3' transferase activity, and 3'-5' nuclease 
activity for error correction. If you spike Taq with 1/100 pfu you keep the 
high 'processiveness' of Taq and gain the editing function of pfu. 

ASHG




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