pcr taq pfu
pennie at access2.digex.net
Tue Jan 31 15:23:39 EST 1995
On 26 Jan 1995 goldman%bchem.dnet at DXI.NIH.GOV wrote:
> Have tried pfu in straight pcr. it is a very unreliable and fussy enzyme,
> but has the advantage of NO 3' transferase activity, and 3'-5' nuclease
> activity for error correction. If you spike Taq with 1/100 pfu you keep the
> high 'processiveness' of Taq and gain the editing function of pfu.
Would someone explain the kinetics of this to me. Surely you would need
more than 1/100 (are we talking units here) in order to ensure taq wasn't
proceding too far after an error before there was a chance of pfu getting
on to proofread, or am I missing something (again).
pennieb at dce41.nci.nih
pennie at access.digex.net
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