DNA extraction

HK hk-miami at ix.netcom.com
Mon Nov 6 21:44:49 EST 1995


In <47fo1j$1h1 at lyra.csx.cam.ac.uk> Ricky Critcher
<rc at mole.bio.cam.ac.uk> writes: 
>
>Does anybody know of a method to extract DNA from cells treated for 
>metaphase spreads by the standard method and stored in fix (3:1
methanol 
>to acetic acid).
>
>Cheers 
>
>
Ouch!!!  The DNA would be destroyed by the acid in the fixative.  You
need to harvest your chromosomes and store them in ethanol.  Never
fix!!!
HK



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