Long range PCRs for sequencing

Hoang Trang hoang.trang at utoronto.ca
Wed Nov 8 03:45:27 EST 1995

Barnes in the March 15, 1994 issue of Proceeding of the National Academy 
of Science has a good discussion on long range PCR that may be useful.  
In it he compared several parameters relating to long range 
amplification.  I am trying to amplify a 2 kb human genomic fragment with 
varying degrees of success.  Any suggestion with regards PCR reagents 
concentration and cycling conditions that you have had success with or 
any papers that you have come across would be appreciated.

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