Barnes in the March 15, 1994 issue of Proceeding of the National Academy
of Science has a good discussion on long range PCR that may be useful.
In it he compared several parameters relating to long range
amplification. I am trying to amplify a 2 kb human genomic fragment with
varying degrees of success. Any suggestion with regards PCR reagents
concentration and cycling conditions that you have had success with or
any papers that you have come across would be appreciated.
