Metaphase spreads

David D Morgan gen159 at abdn.ac.uk
Wed Oct 18 17:07:06 EST 1995


hk-miami at ix.netcom.com (HK ) wrote:

>In <45thot$hl7 at clus1.ulcc.ac.uk> "g.coulton" <g.coulton at cxwms.ac.uk>
>writes: 
>>
>>Thanks for all your helpful e-mails regarding my request for methods
>for 
>>making metaphase sreads. It would, of course, have helped if I had
>added 
>>that I am working both with human and mouse cells!
>>
>>Thanks once again in advance.
>>
>>Gary Coulton
>>
>>------------------------------------------------------------
>You can do mouse cells the same way as human.  Just experiment around
>abit with colcemid and hypotonic treatment times.  If you are using
>human probes with the mouse chromosomes, you may want to reduce the
>stringency a bit, but I find that this also increases background, and
>have usually had good results using the same stringency conditions as
>for human probes on human chromosomes.

>Are you using fibroblast cultures or lymphocytes?

>Helene


On a similar note can you do "spreads" with S. cerevisiae chromosomes ?

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