Metaphase spreads

HK hk-miami at ix.netcom.com
Thu Oct 19 17:39:30 EST 1995


>>>------------------------------------------------------------
>>You can do mouse cells the same way as human.  Just experiment around
>>abit with colcemid and hypotonic treatment times.  If you are using
>>human probes with the mouse chromosomes, you may want to reduce the
>>stringency a bit, but I find that this also increases background, and
>>have usually had good results using the same stringency conditions as
>>for human probes on human chromosomes.
>
>>Are you using fibroblast cultures or lymphocytes?
>
>>Helene
------------------------------------------------------------------->
>
>On a similar note can you do "spreads" with S. cerevisiae chromosomes
?
>

>* David D Morgan     
>* University of Aberdeen      
>* Aberdeen, SCOTLAND    
>* Tel: 01224 273105                                                   
 *>* Fax: 01224 273144     
>* Email: gen159 at abdn.ac.uk                             



That's a real tough one.  From the minimal literature, it appears to be
possible with meiotic chromosomes, rather than mitotic ones.  The
trick, then, is to get the little buggers to sporulate.  I tried a few
protocols, but either didn't get it to work, or it did, but I just
didn't recognize it under the scope. Don't have the sources handy...I'm
at home, and they're in the lab.  I have since moved on to other
projects.  I'll take a look tomorrow, and see if I can put my hands on
the sources.

Helene



More information about the Biochrom mailing list