What is a good DAPI concentration for F.I.S.H. ?
dellaire at ODYSSEE.NET
Mon Apr 29 07:34:14 EST 1996
I just bought a new stock of DAPI and I have no idea what would be a
good concentration for FISH (i.e. counterstaining chromosomes).
Does anyone one have a good rule of thumb... ie. for time and conc.
As well, what is a good solvent to disolve it in? I think the old stock
we had was in PBS... but I am not sure... I have heard others disolve
the DAPI in whatever anti-fade reagent they are using.
Any and all suggestions are welcome.
dellaire at odyssee.net
or popa0206 at po-box.mcgill.ca
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