96 E.coli mini-preps at a time?
matt at AURORA.XO.COM
Thu Aug 22 23:01:46 EST 1996
Thank you for reading this.
What we are looking for is a protocol for doing 96 minipreps of
transformed E.coli at one time. We would like to start with at least
1.5 ml of ON culture and end up with about 50ug plasmid DNA. We are
using a 2micron plasmid and the E.coli strain DH10B. The DNA from the
minipreps will be used for sequencing and tranforming of E.coli and
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