96 E.coli mini-preps at a time?

Matt Ashby matt at AURORA.XO.COM
Thu Aug 22 23:01:46 EST 1996


Thank you for reading this.

What we are looking for is a protocol for doing 96 minipreps of 
transformed E.coli at one time.  We would like to start with at least 
1.5 ml of ON culture and end up with about 50ug plasmid DNA.  We are 
using a 2micron plasmid and the E.coli strain DH10B.  The DNA from the 
minipreps will be used for sequencing and tranforming of E.coli and 
S.cerevisiae.

Thank you,

Doug Gilliland



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