96 E.coli mini-preps at a time?

Peter J. Myler mylerpj at u.washington.edu
Fri Aug 23 12:25:41 EST 1996


We are using a 96-well protocol which we modified slightly from one posted
by Bruce Roe.  You can find his protocol at
http://dna1.chem.uoknor.edu/dsis_seq.html  (Bruce, I hope you don't mind my
broadcasting this).  We have had very good success using this technique and
generally get a reasonable amount of sequencable DNA.

Regards

Peter
 
=====================================================
Peter J. Myler
Seattle Biomedical Research Institute
4 Nickerson Street
Seattle, WA  98109-1651
phone: (206) 284-8846x332
FAX: (206) 284-0313
e-mail: MYLERPJ at U.WASHINGTON.EDU
=====================================================

Matt Ashby <matt at AURORA.XO.COM> wrote in article
<321D2E2F.E67 at mendel.berkeley.edu>...
> Thank you for reading this.
> 
> What we are looking for is a protocol for doing 96 minipreps of 
> transformed E.coli at one time.  We would like to start with at least 
> 1.5 ml of ON culture and end up with about 50ug plasmid DNA.  We are 
> using a 2micron plasmid and the E.coli strain DH10B.  The DNA from the 
> minipreps will be used for sequencing and tranforming of E.coli and 
> S.cerevisiae.
> 
> Thank you,
> 
> Doug Gilliland
> 
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