Mouse Chromosome Paints??? DO they exits?

coco coco at nki.nl
Tue Feb 6 12:03:24 EST 1996


In article <4euns3$7bl at sifon.cc.mcgill.ca>, popa0206 at PO-Box.McGill.CA says...
>
>Doses anyone out there have chromosome specific coatasomes (chromosome 
paints) for the mouse
>Or know of a reference for them....
>
>
>I have been trying to find some in the literature and I am having a hard 
time.
>
>
>Thanks in advance
>
>Graham Dellaire
>
>dellaire at odyssee.net


Yes the do exist. Here is the reference:

Nat Genet 9: 369-375 (1995)[95315987] 

Chromosome specific paints from a high resolution flow karyotype of the
mouse.

P. Rabbitts, H. Impey, A. Heppell-Parton, C. Langford, C. Tease, N. Lowe, D. 
Bailey, M. Ferguson-Smith & N. Carter

MRC Radiotherapeutics Unit, MRC Centre, Cambridge, UK. 

Chromosomes from antigen stimulated B-cells from spleens of inbred mice have 
been separated using flow cytometry into 18
distinguishable peaks. Using locus-specific oligonucleotides and fluorescence 
in situ hybridization to banded metaphase
spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 
12, 16, 17, 18, 19, X and Y. The remaining six
chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 
with 15, were resolved by flow sorting
chromosomes from mice carrying an appropriate homozygous translocation and 4, 
5 and 14 have been isolated in this way.
This is the first demonstration of how a complete set of mouse chromosome 
paints can be produced. 


Jose 

 




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