Telomeres and chromosome lo

David Matthes matthes at BIOMAIL.SJSU.EDU
Wed Feb 14 20:10:08 EST 1996


Telomeres and chromosome loss in hybridomas
In my reading about the formation of hybrids between human fibroblasts or 
lymphocytes and mouse tumor cell lines, I encounter little in the way of 
explanation for the unequal chromosome loss in the hybrid cells.  I 
recognize that it is advantageous for mapping genes to human chromosomes 
that most (but not all) of the human chromosomes are lost, but my 
question is why are the human chromosomes lost and not those of the mouse?

I only know of the following two speculations:

1) Mouse chromosomes may replicate more quickly than human chromosomes.  
Failure to replicate as quickly results in loss.  

2) The chromosome loss happens in a similar way as occurs in haploidization 
in Aspergillis.  I'm not sure why or how this would account for the species 
bias of chromosome loss, but it was mentioned by one author.

Does anyone know of work examining how differences in telomeres and 
telomerase in the two parent cell lines might contribute to the differential 
chromosome loss?   

Have reciprocal fusions been made in which human tumor lines have been fused 
with mouse fibroblasts or lymphocytes?  Are mouse chromosomes lost in such 
hybrids? 

Thank you.






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