James Holman <J.Holman at botany.uq.edu.au> wrote:
>Hi everyone,
>I currently trying to carry out chromosome counts on Senna (small shrub)
>but have encountered problems. I am doing root tip squashes. One
>problem is that the roots are very fibrous and thin thus causing it to be
>difficult to macerate.
I had to do root tip squashes for c-banding as a project student, and found
that softening the cell wall by partly digesting the cellulose made a big
difference. I'm afraid I don't have the reference any more, but it was from a
paper studying the c-banding of chromosomes of allium species. Another method
was to completely digest the cell wall but that involves centrifugation and so
uses more equipment.
Jo
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