FISH on blood smears

Riggs Penny rpenny at Bayou.UH.EDU
Tue Nov 12 17:13:19 EST 1996


ricky critcher (rc at mole.bio.cam.ac.uk) wrote:


: I am trying to FISH yacs onto mouse peripheral blood smears but so far
: have had no success. Does any one out there have some handy hints. Any
: help would be greatly appreciated.

Try treating the blood sample with 0.75M KCl for 10 min, centrifuge
and remove most of the supernatant, then make a "smear" with a few
drops of the remaining liquid/pellet.  This method will allow you
to get rid of most of the red blood cells so you can hybridize to
the remaining leucocyte nuclei.

Penny Riggs
                                      NASA LB Johnson Space Center 
rpenny at bayou.uh.edu                   Mail Code SD2
http://www.sss.org/~penny             Houston, TX 77058









More information about the Biochrom mailing list