Subcloning lambda DNA

griffen at CGL.UCSF.EDU griffen at CGL.UCSF.EDU
Tue Oct 22 12:01:06 EST 1996

I am trying to subclone fragments from a lambda dash clone pulled from a
mouse genomic library into bluescript. The fragments are between 7 and
13 kb with sticky ends, and i have been purifiying them by galssmilk
based methods, or by electroelution, and ligating using the clonetech
high efficiency ligation kit. So far i have had no success, and am very
frustrated. Any suggestions welcomed.

Maria Wilson

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