Eukaryotic mRNA Intron/Exon Boundary Consensus Sequences?
Andy.Law at bbsrc.ac.uk
Mon Sep 9 03:42:55 EST 1996
In article <50til3$6v8 at nntp3.u.washington.edu>, roach at u.washington.edu
(Jared Roach) wrote:
> Can anyone point me to a good reference?
> All I know is that functional introns usually begin with GT
> and end with AG. Are there known exceptions? How rare are
> they? In short, given a random intron, what is the probability
> distribution for the composition of its first and last two
> base pairs?
Try the following as a starting point. Use ENTREZ to look them up and find
related papers. There are probably more up-to-date references available.
Jackson, I.J. (1991) A reappraisal of non-consensus mRNA splice sites.
Nucleic Acids Research 19, 3795-3798.
Mount, S.M. (1982) A catalogue of splice junction sequences. Nucleic Acids
Research 10, 459-472.
Ohshima, Y. & Gotoh, Y. (1987) Signals for the selection of a splice site
in pre-mRNA: Computer anaysis of splice junction sequences and like
sequences. Journal of Molecular Biology 195, 247-259.
Shapiro, M.B. & Senapathy, P. (1987) RNA splice junctions of different
classes of eukaryotes: Sequence statistics and functional implications in
gene expression. Nucleic Acids Research 15, 7155-7174.
> Thanks, Jared
> EXTRA BONUS QUESTION: Does this probability distribution
> depend on the species?
( Andy.Law at bbsrc.ac.uk )
( Big Nose in Edinburgh )
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