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mapping large segments

Joe Miller jtmille1 at STUDENTS.WISC.EDU
Thu May 29 14:49:14 EST 1997


	I am working on a 100kb BAC fragment and have subcloned several
repetitive and single copy sequences (all less than 3 kb).  I would like to
make a restriction map of the entire BAC.  Other than subcloning smaller
fragments (e.g. 10-50 kb) which could take a long time to dissect and order
does anyone have any suggestions for making a broad baseline map of the
entire BAC.

Thanks

Joe Miller
Department of Horticulture
University of Wisconsin-Madison





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