Long primers for PCR
Wellcome Trust, Nairobi.
wellcome at USERS.AFRICAONLINE.CO.KE
Sat Nov 8 08:39:56 EST 1997
If there any risk of amplifying a fragment DNA with primer which are 50
to 60 pb. Actually, I want to introduce a enzyme restriction site in
these primers so that I can easily detected them in agore or Acrylamide
gel. Therefore I do need longer primers. But I wandering about doing a
PCR such long oligonucleotides.
Wellcome Trust Research Labs,
PO Box 43640,
Tel 254 (2) 725390, Fax 254 (2) 711673
E-mail: wellcome at users.africaonline.co.ke
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