CAPS markers won't show heterozygotes

[Mike Cooley]

Hi, I am doing map-based cloning in Arabidopsis and twice now I have
developed CAPS markers only to find that they will not distinguish the
heterozygotes from one of the parents. Assuming that maybe my "known"
heterozygous individual were not really heterozygous I mixed DNA's from
the two parents to "create" a heterozygous DNA and still it came up
homozygous for one of the parents (it is always the same parent). I do
not understand how this occurs. Any ideas?

--
Dr. Michael Cooley
Waksman Institute
Rutgers University
190 Frelinghuysen Rd
Piscataway, NJ 08854

cooley at mbcl.rutgers.edu
phone 732-445-3802
FAX 732-445-5735