Sequenzing of BACs

Henning Gohlke h.gohlke at gsf.de
Fri Nov 13 05:15:26 EST 1998


Hello everybody!
I am doing a positional cloning approach to identify a cataract mutaion
in mice. I have a lot of trouble with sequencing the Bacs. I am using a
modified Qiagen-Bac-Preparation. I very often get a good harvest, but I
am not always able to sequence it. Can anyone give my an advice, how I
can improve the qualtity of my Bac-Preparation.
Thank you very much
Yours Henning




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