double signal in chromos. FISH ?
hohoff at uni-muenster.de
Fri Mar 19 07:34:13 EST 1999
Dear experts in the field of chromosomal FISHing !
I expected a double-signal on metaphase chromosomes (due to the occurrence
of the gene on both chromatides) - but what I observe in nearly half of the
chromosomes inspected is that there is just ONE fluorescence signal !
What may be the reason ? Is the chromatide DNA not denatured well enough ?
Any helpful comment is appreciated
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