identiication of rDNA

[MARCEL QUINT]

Dear colleagues,
I want to check plasmid inserts in a Southern HYB for clones with
repetitive sequences. So I am planning to hybridyze with maize
genomic DNA to identify these rDNA containing clones. I am not
sure about the amount of labelled probe to use for this HYB. For a
normal Southern with a certain fragment as probe i use 50-100ng
(P32-labelling). But is this enough to cover all species of rDNA
within genomic DNA?
Thanks for your answers in advance,

Marcel


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Marcel Quint

Universität Hohenheim
Institut für Pflanzenzüchtung, Saatgutkunde und Populationsgenetik
Fruwirthstrasse 21
70593 Stuttgart
Tel# 0711/459-2697 o. -3491
Fax# 0711/459-2343

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