identiication of rDNA

MARCEL QUINT Quint at 350-ipsp.ipsp.Uni-Hohenheim.DE
Thu Nov 16 04:19:08 EST 2000

Dear colleagues,
I want to check plasmid inserts in a Southern HYB for clones with
repetitive sequences. So I am planning to hybridyze with maize
genomic DNA to identify these rDNA containing clones. I am not
sure about the amount of labelled probe to use for this HYB. For a
normal Southern with a certain fragment as probe i use 50-100ng
(P32-labelling). But is this enough to cover all species of rDNA
within genomic DNA?
Thanks for your answers in advance,



Marcel Quint

Universität Hohenheim
Institut für Pflanzenzüchtung, Saatgutkunde und Populationsgenetik
Fruwirthstrasse 21
70593 Stuttgart
Tel# 0711/459-2697 o. -3491
Fax# 0711/459-2343



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