Here's a question to ponder...
What is a biofilm anyway? Is one attached cell a biofilm? 100?
1000000? A monolayer? Is a 3-D structure a requirement for a
"biofilm"? Perhaps an attached population becomes a biofilm when the
activity of the population is different from the sum of the
A form of "synergy"? Well, if the characteristics of cells change
immediately upon attachment (a physiological response to being
attached such as increased resistance to antimicrobials), then perhaps
a biofilm could be one attached cell ("a biofilm onto itself")
Is this fun or what?