Extraction of biosurfactants

Andrew Singer asinger at MAIL.UCR.EDU
Tue Jun 3 18:10:23 EST 1997


I am interested in the subject of biofilms as it pertains to the microbial
production of surfactant-like molecules.  I was wondering if anyone had any
experience with extracting unknown surfactants from a pure culture.  I have
found numerous references to studies where people have extracted
surfactants, however, "they" knew what they were looking for, whereas I
don't. Which is where my second question comes in.  If I can do a quick
screening assay for what "kind" of surfactant it is, I can probably find an
extraction assay which will suit my purposes...however, I would still be
most appreciative of any suggestions regarding an extraction procedure, as
well.  Comments/answers?

Thanks!!!

Andrew Singer					
Department of Soil and Environmental Science
University of California at Riverside
Riverside, CA  92521		
asinger at mail.ucr.edu		 

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