Area/Space covered by bacteria in colonies/biofilms

paul stoodley P.Stoodley at exeter.ac.uk
Thu Sep 25 06:09:38 EST 1997


In response to Jan Kreft's question:

A while back I was doing some work with Dirk deBeer in 
which we sucked biofilm clusters out of a biofilm 
using a micropipette and enumerated the viable cells 
by plate count. The clusters were approx 200 microns 
diameter. We estimated the volume of the clusters by 
assuming they were hemispheres. We got about 1x10^10 
cells / ml of cluster material. The cells were gram 
-ve rods 2 microns long by 1 micron wide to give a 
vol of 1.31 microns cubed. This works out that the vol 
occupied by cells in a cluster is 1.31 %. Some of this 
is written up in Stoodley, P., de Beer, D., and 
Lewandowski, Z. 1994. "Liquid flow in biofilm 
systems." Applied and Environ. Microbiology. 
60:2711-2716. Also included in this paper is how the 
surface covered by a biofilm varies with depth.

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----------------------
Paul Stoodley

Environmental         Tel: 01392 264348       
Microbiology          Fax: 01392 263700
Research              email: p.stoodley at exeter.ac.uk
Group
Exeter University

Biological Sciences
Hatherly Laboratories
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Exeter EX4 4PS. UK.




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