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"Tagging" live bacteria

Mark fuller at envirogen.com
Sun Apr 19 03:23:41 EST 1998


Collegues,

I am attempting to "tag" bacteria for subsequent injection into both
sediment cores and an aquifer.  I want to be able to detect the injected
bacteria in groundwater samples taken at set distances from the
injection point.  The bacteria will most likely not be growing in the
first use, but may be in other instances.  Of course, for the field
injections, we cannot use radioactive materials, nor can the organisms
be genetically engineered (but may be later on).  There is also a
background of indigenous organisms at the site (about 10^5 CFU/ml).

My question is what could be used to tag the bacteria without killing
them and that would last for up to 20 days in the cells?  Of the
following, what recent experience/literature is available:

immunoassay
DNA-probes
fluorescent staining of cells (non-nucleic acid)


Any and all advice or suggestions would be greatly appreciated.


Mark Fuller
fuller at envirogen.com


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