high-throughput screening for antimicrobial proteins

xcheng at oreo.science.uottawa.ca xcheng at oreo.science.uottawa.ca
Mon Dec 7 07:24:48 EST 1998

Dear Netters,

I am looking for  high-throughput methods for isolating 
gene coding for previously unknown  antimicrobial or antibacterial 
proteins (peptides) from different sources. Routine methods involve 
purification of tentative antimicrobial proteins from different 
sources and test of the antimicrobial activity of the purified 
proteins with different microbial inhibition methods, and cloning of 
the genes using molecular biology method.  Due to the tedious process 
in protein purification and vast number of proteins available, this 
routine method could be highly inefficient in cloning of an novel  
antimicrobial gene . Are you aware of any method which can identify 
the antimicrobial genes in a straightforward way? 

I appreciate very much of your input in my search efforts.

Xiongying Cheng

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