Particles for size calibration

Jan Kreft Kreft at cardiff.ac.uk
Sat Oct 24 04:03:25 EST 1998


Dear all,

I'm recording cell growth in a flow cell and feel that calibration of the
size measurements by image analysis is important.

Most people use fluorescent latex beads for calibration of epifluorescence
microscopes.

I've tried latex and silica beads with a phase contrast microscope, but
they are phase-bright in contrast to the phase-dark bacteria. Since the
optical properties are so different, I don't think that the edge detection
algorithm will give the same results for bacteria and latex beads of the
same size.

Has anyone an idea of what particles of known size are about as
phase-dark as bacteria?

Thanks,

Jan.

Dr. Jan-Ulrich Kreft			Phone +44 (0)1222 876036
Cardiff School of Biosciences		Fax   +44 (0)1222 874305
Cardiff University			E-mail Kreft at cardiff.ac.uk
PO Box 915, Cardiff CF1 3TL, UK



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