fatty acid extraction

[fuller at envirogen.com]

Fellow scientists,

I am working with bacterial fatty acids, and am quite familair with the
literature.  However, I was wondering if anyone has heard of new,
alternative methods for total fatty acid extraction.  I've been using
the one-phase chloroform:methanol:buffer method, but I'd like to see if
there is an alternative, maybe with a less-dense-than-water solvent
which could be reovered without the use of a sep funnel.

Any leads or refs much appreciated.

Thanks.

-Mark
fuller at envirogen.com


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