Method of Quantification for biofilms.

Ian Goldberg naig at ihug.co.nz
Thu Mar 25 03:52:33 EST 1999


Hi,
I am starting a project in biofilms and I want to begin by quantitating the 
effect of various environment conditions (ie. temp, nutrients) on a single 
species biofilm. Is there a standard method for doing this? Is the Lowry 
method of protein quantification scientifically acceptable? Has anyone 
trying the crystal violet method described by O'Toole, G. A., and R. Kolter. 
1998. Flagellar and twitching motility are necessary for Pseudomonas 
aeruginosa biofilm development. Mol. Microbiol.30:295-304?
I would appreciate any feedback.
K. Matejka






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