Microbial EPS

Harry Bryant hebryant at scj.com
Tue Jul 3 11:06:01 EST 2001


There is a very good little book that I don't think Tony mentioned. I
think it will answer most of your questions.


Title:  Microbial Extracellular Polymeric Substances:
Characterization, Structure and Function
Eds: Jost Wingender, Thomas Neu, and Hans-Curt Flemming
Copyright: 1999
ISBN: 3-540-65720-7
Publisher: Springer-Verlag



Tony_Rook at steris.com ("Rook, Tony") wrote in message 
news:<9hqi5f$ra$1 at mercury.hgmp.mrc.ac.uk>...
 > Mr. Tiyawisutsri:
 >
 > There is a plethora of research in the area of the biofilm matrix otherwise
 > referred to a the bacterial glycocalyx.  Here are some pertinent references
 > in this area (although there is much more):
 >
 >
 > 1.      Costerton JW, Irvin RT, Cheng KJ.  The Bacterial Glycocalyx in
 > Nature and Disease.
 >          Ann. Rev. Microbiol.  1981.  35: 299-324.
 >
 > 2.      Marshall KC.  Mechanisms of Bacterial Adhesion at Solid-Water
 > Interfaces.  In: Bacterial Adhesion:  Mechanisms and      Physiological
 > Significance (eds.  Savage DC, Fletcher M).  Plenum Press, New
 > York.  1985.  pp 133-161.
 >
 > 3.      Sutherland IW.  Microbial Exopolysaccharides - Their Role in
 > Microbial Adhesion in Aqueous Systems.  CRC Critical       Reviews in
 > Microbiology.  Volume 10, Issue 2: 173-201.
 >
 > 4.      Allison DG.  Biofilm-Associated Exopolysacharides.  Microbiology
 > Europe.  Nov/Dec 1993. pp 16-19.
 >
 > As for your specific questions:
 >          1. How to distinguish bacterial exopolysaccharide from biofilm
 > exopolysaccharide?
 >
 >                  In the first above reference, Costerton et al defines the
 > bacterial glycocalyx as "those
 > polysaccharide-                        containing structures, of bacterial
 > origin, lying outside the integral elements of the outer membrane of
 > Gram-          negative cells an the peptidoglycan of Gram-Positive cells"1.
 >
 >          2. Is EPS a minor component of biofilms?
 >
 >                  There are several studies which have investigated the
 > exopolysaccharide content of biofilms.  These should
 > give                 you some idea of the content ratios of EPS to other
 > biofilm components such as bacterial cells.
 >
 >          3. Are there specific biofilm exopolysaccharides?
 >
 >                  Although there are major polysaccharide groups involved in
 > biofilm formation, much of the specific EPS                  composition is
 > dependent on the microorganisms within the biofilm that is generating the
 > EPS.  The composition          and ratios of different exopolysaccharide
 > produced are not only dependent of the specific bacterial
 > cell                        producing them but is also influenced by
 > physical conditions ( i.e. influence of substratum, flowing
 > conditions,                and available nutrients).  Much of this is
 > detailed in the cited references listed above.
 >
 > Hope this helps,
 >
 > Tony A. Rook
 > Scientist
 > STERIS Corporation
 > 5960 Heisley Road
 > Mentor, OH 44060  USA
 > Phone: (440) 392-7743
 > Email:  tony_rook at steris.com
 >
 >
 > -----Original Message-----
 > From: "rachaneeporn tiyawisutsri" [mailto:rachaneeporn_t at hotmail.com]
 > Sent: Monday, July 02, 2001 5:15 AM
 > To: biofilms at net.bio.net
 > Subject: (NONE)
 >
 >
 >
 > Hello all, I am a graduate student in Medical technology at Mahidol
 > University. I will have a seminar in " The biofilm matrix" . Please suggest
 > me about the content. And I have some question about " How to distinguish
 > bacterial exopolysaccharide from biofilm exopolysaccharide ?" ,
 > Exopolysaccharide is the minor component of the bacterial biofilm? and the
 > last question " Are there any specific biofilm exopolysaccharide ?
 > Thanks for help
 >
 > Rachaneeporn Tiyawisutsri
 > _________________________________________________________________________
 > Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.
 > ---
 >
 >
 >
 >
 >
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