Biofilm staining

Rook, Tony Tony_Rook at steris.com
Wed Jun 20 09:25:31 EST 2001


Dear Dr. Shapiro:

Check out these references, I think they might help.

         Bayston R, Rodgers J.  Production of extra-cellular slime by 
Staphylococcus epidermidis during stationary phase of      growth:  its 
association with adherence to implantable devices.  J of Clin 
Pathol.  1990.  43:866-869.

         Whiteman P.  The quantitative measurement of alcian blue - 
glycosaminoglycan complexes.  Biochem J. 1973. 131:  343-50.


Both of these use an alcian blue staining method to estimate EPS.  Hope 
they help.

Good Luck,

Tony Rook
Scientist
STERIS Corporation
Mentor, OH USA
tony_rook at steris.com

-----Original Message-----
From: Dr. S. Shapiro [mailto:toukie at zui.unizh.ch]
Sent: Monday, June 18, 2001 3:27 AM
To: biofilms at net.bio.net
Subject: Biofilm staining


Dear Colleagues;

       I remember reading a while back that people have quantified biofilms
in glass or plastic vessels by first aspirating the planktonic cells, then
staining the adherent biofilm cells with a dye, and then measuring the
amount of dye, either as a thin film or following solubilisation.  However,
I have been unable to locate any such references.  If anyone is aware of
such references, would you be kind enough to contact me _directly_ at

toukie at zui.unizh.ch

with a list of relevant citations?

Thanks in advance to all responders,

S. Shapiro
toukie at zui.unizh.ch
jk

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