12 Nov seminar on live cell imaging

Anya Salih anya at emu.usyd.edu.au
Thu Oct 31 19:13:30 EST 2002


There will be a seminar on live cell imaging on Tues 12th Nov, followed by 
wine and other refreshments.

Venue: Room LG92, Electron Microscope Unit (Madsen Building), University of 
Sydney

<http://www.emu.usyd.edu.au/images/unimap.html>

Time: 16:00-17:00

LIVE CELL IMAGING: FROM LIGHT TO PROBE MICROSCOPY

Dr Filip Braet

Laboratory for Cell Biology and Histology, Free University of Brussels 
(VUB), Laarbeeklaan 103, 1090 Brussels-Jette, Belgium

Scientists have long been interested in live cell imaging using traditional 
light microscopic imaging methods. At that time, they were unable to 
identify organelles, molecules or biochemical processes at the 
(sub)cellular level. Within the last decade, however, combinations of novel 
microscopic techniques with advanced electronic imaging and digital image 
processing technology have resulted in dramatic improvement of the quality 
of microscopic images, providing high-quality images or movies with a 
resolution close to, or even below the theoretical limit of resolution. 
Another major improvement is the recent availability of numerous 
fluorescent or non-fluorescent probes specially designed for close in vitro 
and in vivo monitoring of living cells. Today, molecules, proteins or 
organelles hitherto invisible, can be clearly observed and measured, and 
the recording of dynamic events, including molecular processes within 
living cells has become routinely feasible. In the present talk we will 
present a survey of the obligate conditions to be implemented during life 
cell imaging over considerable periods of time: i.e., maintenance of 
steady-state culture conditions regarding temperature, pH, CO2 and 
osmolarity. The choice of optics, recording media and the use of contrast 
methods together with the application of bioprobes will be discussed. 
Finally, viability assessment of the cells during imaging remains an issue. 
During the course of the presentation, the following microscopic methods 
for cellular imaging will be outlined: time-lapse video microscopy, in vivo 
microscopy (IVM), time-lapse (video) fluorescence microscopy, confocal 
laser scanning microscopy (CLSM), and atomic force microscopy (AFM). 
Accordingly, we will address some pronounced problems and artefacts 
encountered during life cell imaging for each microscopic method presented. 
In conclusion, the presented microscopical methods and conditions for life 
cell imaging is not intended to be complete, but it comprises a selection 
of examples to illustrate and summarize the basic strategies for their 
application and their usefulness in the field of life sciences. Nowadays, 
the modern microscope for life cell imaging is an indispensable tool in any 
biological or biomedical laboratory interested in the structure and 
dynamics of the living cell.

-- 
Dr Anya Salih
APD(I) Research Fellow
Electron Microscope Unit
& The Australian Key Centre for Microscopy & Microanalysis
Madsen Building FO9 Email: anya at emu.usyd.edu.au
The University of Sydney Telephone: 02-93517540
Sydney, 2006, AUSTRALIA Facsimile: 02-93517682
http://www.emu.usyd.edu.au/research/anya_research.html


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