[Biofilms] staining of bacterial biofilms

Bob McLean via biofilms%40net.bio.net (by mclean from txstate.edu)
Tue Jun 19 14:48:29 EST 2007


Dear Rachna,

While in theory any stain that would stain biological components of 
biofilms (and not the underlying polystyrene substratum) could be 
used, crystal violet absorbs much more light than safranin and so 
would provide higher sensitivity for this assay.

Good luck with your work,

Bob McLean

At 09:15 AM 6/19/2007, Rachna Singh wrote:
>Why is crystal violet preferred over safranin for staining bacterial
>biofilms formed on the surface of microtiter plates?
>
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R.J.C. (Bob) McLean
Department of Biology
Texas State University
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USA

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(512) 245-3365 phone
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Email: McLean from txstate.edu

Website: http://www.bio.txstate.edu/~micro/mclean/mclean.html 



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