[Biofilms] Re: staining of bacterial biofilms

Rachna Singh via biofilms%40net.bio.net (by rachna.mbio from gmail.com)
Wed Jun 20 07:07:34 EST 2007


On Jun 20, 12:48 am, Bob McLean <mcl... from txstate.edu> wrote:
> Dear Rachna,
>
> While in theory any stain that would stain biological components of
> biofilms (and not the underlying polystyrene substratum) could be
> used, crystal violet absorbs much more light than safranin and so
> would provide higher sensitivity for this assay.
>
> Good luck with your work,
>
> Bob McLean
>
> At 09:15 AM 6/19/2007, Rachna Singh wrote:
>
> >Why is crystal violet preferred over safranin for staining bacterial
> >biofilms formed on the surface of microtiter plates?
>
> >_______________________________________________
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>
> R.J.C. (Bob) McLean
> Department of Biology
> Texas State University
> 601 University Drive
> San Marcos, TX 78666
> USA
>
> New University Name: Texas State University
>
> (512) 245-3365 phone
> (512) 245-8713 FAX
> Email: McL... from txstate.edu
>
> Website:http://www.bio.txstate.edu/~micro/mclean/mclean.html

OK. Thanks a lot for your help.




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