[Biofilms] Biofilm experiment questions

[merhawi debesai]

Hi,
  Thank you so much for your information in advance.
  Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions:
   
   1. On QCM-D data interpretation: 
      i. frequency change  --> adsorbed mass
      ii. energy of dissipation --> to visico-elastic property of the adsorbed 
          mass. 
  2. The second question is regarding the preparation of bacterial strains for 
       surface analysis. 
     i. Usually, we prepare the test strains in suspensions. However, it is   
        known that there....difference between planktonic and sessile (biofilm)  
        bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?!
   
  Sincerly yours,
  tesfalem

       
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